Description of the Internship:
We are preparing new synthetic viruses based on AAV to be specific pharmacologic treatments for a number of human complaints. Part of our team has just finished the construction of one such virus that should only function in Thyroid cells (hopefully with a preference to Thyroid cancer) after 5-6 years of work. This was the main project of one of our Graduate students (PhD), who has just finished her graduate work. Now we would like to check the specificity of this virus in many types of human cell cultures, of thyroid origin as well as other tissues. Our intern's work will involve carrying out this study with a version of this virus that upon efficient infection will turn the infected cell green under FITC light. That is Thyroid cells should turn green, cells of other tissue origins should be their natural color. The work will entail preparing batches of this virus, determining their functional concentration, preparing primary cell cultures from human tissues and from human cell lines, infecting them with this and control virus (in a protected manner) and then following the infection to determine which cells gets infected and how well.